Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907-1165.
Plant Physiol. 1992 Sep;100(1):409-15. doi: 10.1104/pp.100.1.409.
Accumulation of both osmotin mRNA and osmotin protein in tissues of tobacco (Nicotiana tabacum L. var Wisconsin 38) is subject to complex developmental control. Osmotin was found to be most abundant in tobacco roots and in tissues of the outer stem comprised primarily of epidermis, and it was less abundant in the corolla. It was a minor protein in other tissues and was undetectable in some tissues, including those of developing and mature seeds. The mRNA abundances did not always reflect the amount of protein accumulation because in some tissues high levels of mRNA but not protein were measured and vice versa. Accumulation of osmotin mRNA but not protein occurred in some plant tissues due to treatment with abscisic acid, wounding, and tobacco mosaic virus infection. Ethylene induced the accumulation of osmotin mRNA and, to a small extent, protein in seedlings, but was ineffective with cultured cells. Exposure of cultured cells and plants to NaCl caused high levels of both mRNA and the protein to accumulate. Thus, the accumulation of osmotin mRNA is controlled developmentally and by at least five hormonal or environmental signals. However, posttranscriptional processes can limit osmotin accumulation.
在烟草（Nicotiana tabacum L. var Wisconsin 38）组织中，渗透素 mRNA 和渗透素蛋白的积累受到复杂的发育调控。渗透素在烟草根和主要由表皮组成的外茎组织中最为丰富，在花冠中则较少。它在其他组织中是一种次要蛋白质，在一些组织中无法检测到，包括发育中和成熟的种子组织。mRNA 的丰度并不总是反映蛋白质积累的程度，因为在一些组织中，测量到高水平的 mRNA 但没有蛋白质，反之亦然。由于脱落酸、创伤和烟草花叶病毒感染的处理，一些植物组织中渗透素 mRNA 的积累，但没有蛋白质。乙烯诱导幼苗中渗透素 mRNA 的积累，在较小程度上诱导蛋白质的积累，但对培养细胞无效。培养细胞和植物暴露于 NaCl 会导致高水平的 mRNA 和蛋白质积累。因此，渗透素 mRNA 的积累受到发育和至少五种激素或环境信号的控制。然而，转录后过程可能会限制渗透素的积累。
