Center for Plant Environmental Stress Physiology, Horticulture Department, Purdue University, West Lafayette, Indiana 247907-1165.
Plant Physiol. 1992 May;99(1):329-37. doi: 10.1104/pp.99.1.329.
A peptide (molecular mass 50 kilodaltons) that is immunologically related to tobacco osmotin was detected in cells of the halophyte Atriplex nummularia. This protein was constitutively expressed in both unadapted and NaCl-adapted cells. A predominant osmotin-like peptide (molecular mass 24 kilodaltons) was also found in culture media after cell growth. Two unique A. nummularia cDNA clones, pA8 and pA9, encoding osmotin-like proteins have been isolated. The pA8 and pA9 inserts are 952 and 792 base pairs and encode peptides of 222 and 224 amino acids, respectively. The peptide deduced from pA8 has a molecular mass of 23,808 daltons and theoretical isoelectric point of 8.31, whereas the peptide derived from pA9 has a molecular mass of 23,827 daltons and an isoelectric point of 6.88. Unique transcripts were detected by the inserts of the cDNA clones, two (1.2 and 1.0 kilobases) by pA8 and one (0.9 kilobase) by pA9. The pA8 transcripts were constitutively accumulated in unadapted and NaCl-adapted cells, whereas the mRNA levels were up-regulated by abscisic acid treatment. The level of pA9 mRNA was induced by NaCl treatment and increased in cells as a function of NaCl adaptation. Southern analysis of the genomic DNA indicated the presence of osmotin-like multigene families in A. nummularia.
在盐生植物滨藜（Atriplex nummularia）的细胞中检测到一种与烟草 osmotin 免疫相关的肽（分子量 50 千道尔顿）。这种蛋白质在未适应和 NaCl 适应的细胞中均持续表达。在细胞生长后，还在培养基中发现了一种主要的 osmotin 样肽（分子量 24 千道尔顿）。已经分离出两种独特的滨藜 cDNA 克隆 pA8 和 pA9，它们编码 osmotin 样蛋白。pA8 和 pA9 的插入物分别为 952 和 792 个碱基对，分别编码 222 和 224 个氨基酸的肽。从 pA8 推导的肽的分子量为 23808 道尔顿，理论等电点为 8.31，而从 pA9 衍生的肽的分子量为 23827 道尔顿，等电点为 6.88。通过 cDNA 克隆的插入物检测到独特的转录物，pA8 有两个（1.2 和 1.0 千碱基），pA9 有一个（0.9 千碱基）。pA8 转录物在未适应和 NaCl 适应的细胞中持续积累，而 ABA 处理会上调其 mRNA 水平。pA9 mRNA 的水平受 NaCl 处理诱导，并随着细胞对 NaCl 的适应而增加。基因组 DNA 的 Southern 分析表明，滨藜中存在 osmotin 样多基因家族。
Zotero 插件
