Plant Biotechnology Laboratory, Institute of Genetics, Academia Sinica, 100101, Beijing, Peoples Republic of China.
Theor Appl Genet. 1995 Jul;91(2):361-6. doi: 10.1007/BF00220900.
Using the rice salt-tolerant mutant 20 as material, a cDNA library was constructed and two salt-inducible clones, SIR5.5 and SIR8.1, were isolated by differential screening. Homology analysis revealed that the two clones together constituted a chimeric rbcL which encoded a truncated large subunit of Rubisco with 337 amino-acids, plus 64 amino-acids of unknown origin. The expressions of both the normal and the chimeric locus appeared to be developmentally regulated and salt-inducible in shoots of the salt-tolerant mutant 20 and its original variety 77-170. In roots, their expressions were salt-inducible in the salt-tolerant mutant 20 whereas no, or only premature, forms were present in the salt-treated original variety 77-170. Higher concentrations of salt reduced the expressions of both normal rbcL and the chimeric locus. ABA showed no effect on their expression.
利用耐盐突变体 20 作为材料，构建了 cDNA 文库，通过差异筛选分离出两个盐诱导克隆 SIR5.5 和 SIR8.1。同源性分析表明，这两个克隆共同构成了一个嵌合 rbcL，它编码一个截断的 Rubisco 大亚基，含有 337 个氨基酸，加上 64 个未知来源的氨基酸。在耐盐突变体 20 及其原始品种 77-170 的芽中，正常和嵌合基因座的表达似乎都受到发育调控和盐诱导。在根中，耐盐突变体 20 中的表达受到盐诱导，而在盐处理的原始品种 77-170 中，几乎没有或只有过早的形式存在。较高浓度的盐会降低正常 rbcL 和嵌合基因座的表达。ABA 对它们的表达没有影响。
