Shioi Y, Takamiya K
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Nagatsuta, Midoriku, Yokohama 227, Japan.
Plant Physiol. 1992 Nov;100(3):1291-5. doi: 10.1104/pp.100.3.1291.
The composition of chlorophyll-precursor pigments, particularly the contents of monovinyl (MV) and divinyl (DV) protochlorophyllides (Pchlides), in etiolated tissues of higher plants were determined by polyethylene-column HPLC (Y. Shioi, S. I. Beale [1987] Anal Biochem 162: 493-499), which enables the complete separation of these pigments. DV-Pchlide was ubiquitous in etiolated tissue of higher plants. From the analyses of 24 plant species belonging to 17 different families, it was shown that the concentration of DV-Pchlide was strongly dependent on the plant species and the age of the plants. The ratio of DV-Pchlide to MV-Pchlide in high DV-Pchlide plants such as cucumber and leaf mustard decreased sharply with increasing age. Levels of DV-Pchlide in Gramineae plants were considerably lower at all ages compared with those of other plants. Etiolated tissues of higher plants such as barley and corn were, therefore, good sources of MV-Pchlide. Absorption spectra of the purified MV- and DV-Pchlides in ether are presented and compared.
通过聚乙烯柱高效液相色谱法(Y. Shioi,S. I. Beale [1987]《分析生物化学》162: 493 - 499)测定了高等植物黄化组织中叶绿素前体色素的组成,尤其是单乙烯基(MV)和二乙烯基(DV)原叶绿素酸酯(Pchlides)的含量,该方法能够完全分离这些色素。DV - Pchlide在高等植物的黄化组织中普遍存在。通过对属于17个不同科的24种植物的分析表明,DV - Pchlide的浓度强烈依赖于植物种类和植株年龄。在黄瓜和叶芥菜等高DV - Pchlide植物中,DV - Pchlide与MV - Pchlide的比例随着年龄增长急剧下降。禾本科植物在各个年龄段的DV - Pchlide水平均显著低于其他植物。因此,大麦和玉米等高等植物的黄化组织是MV - Pchlide的良好来源。文中给出并比较了纯化后的MV - Pchlide和DV - Pchlide在乙醚中的吸收光谱。