Belanger F C, Rebeiz C A
J Biol Chem. 1980 Feb 25;255(4):1266-72.
It is shown that the protochlorophyllide pool of etiolated higher plants is made up of both monovinyl and divinyl protochlorophyllide. Although the two pigments exhibited similar emission maxima, they were distinguishable by their Soret excitation maxima, which were found at 436 to 437 and 443 to 444 nm, respectively, in ether at 77 K. The two pigments were partially separated on thin layers of polyethylene. They were shown to be accompanied by two unknown fluorescent compounds. The latter were designated compound (E451 F626) and compound (E453 F640) where E refers to the Soret excitation maxima and F to the fluorescence emission maxima of the two unknown compounds. Furthermore, it was shown under several different growth conditions that divinyl protochlorophyllide constituted the major component of the protochlorophyllide pool.
结果表明，黄化高等植物的原叶绿素酸酯库由单乙烯基原叶绿素酸酯和二乙烯基原叶绿素酸酯组成。尽管这两种色素表现出相似的发射最大值，但在77K的乙醚中，它们可通过其Soret激发最大值区分，分别在436至437nm和443至444nm处发现。这两种色素在聚乙烯薄层上部分分离。结果表明，它们伴随着两种未知的荧光化合物。后者被命名为化合物（E451 F626）和化合物（E453 F640），其中E指两种未知化合物的Soret激发最大值，F指荧光发射最大值。此外，在几种不同的生长条件下表明，二乙烯基原叶绿素酸酯是原叶绿素酸酯库的主要成分。
