Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, New York 14853.
Plant Physiol. 1992 Nov;100(3):1326-33. doi: 10.1104/pp.100.3.1326.
R82 (sym-17), a stable mutant of Pisum sativum L. cv Sparkle, is described. The shoot growth of the mutant was less than that of its parent under light or dark growth conditions. Gibberellic acid treatment did not normalize the shoot growth of R82. The mutant had thick and short roots. It formed few nodules, but the specific nitrogenase activity was not affected. R82 produced and contained more ethylene than Sparkle. It also contained more free 1-amino-cyclopropane-1-carboxylic acid than did its parent in both the shoot and the root. The root tip of R82 had a lower activity of ethylene-forming enzyme than that of Sparkle, whereas the whole shoot of R82 had a similar activity. The sensitivity of R82 to exogenous ethylene was not more than that of Sparkle. Exogenous ethylene treatments did not make Sparkle mimic R82, and inhibitors of ethylene biosynthesis or action did not normalize the phenotype of R82. The data suggest that the primary effect of sym-17 is not the enhanced ethylene production.
R82（sym-17）是豌豆稳定突变体，描述如下。在光照或黑暗生长条件下，突变体的茎生长小于其亲本。赤霉素处理不能使 R82 的茎生长正常化。突变体具有粗而短的根。它形成的根瘤较少，但特定的固氮酶活性不受影响。R82 产生并含有比 Sparkle 更多的乙烯。它在茎和根中都含有比亲本更多的游离 1-氨基环丙烷-1-羧酸。R82 的根尖乙烯形成酶活性低于 Sparkle，而整个 R82 的茎具有相似的活性。R82 对外源乙烯的敏感性不超过 Sparkle。外源乙烯处理不能使 Sparkle 模仿 R82，并且乙烯生物合成或作用的抑制剂不能使 R82 的表型正常化。数据表明，sym-17 的主要作用不是增强乙烯的产生。
