山药（Dioscorea opposita）休眠珠芽中内源赤霉素的鉴定
Identification of Endogenous Gibberellins in Dormant Bulbils of Chinese Yam, Dioscorea opposita.
作者信息
Tanno N, Yokota T, Abe M, Okagami N
机构信息
Department of Biology, Faculty of Science, Yamagata University, Yamagata 990, Japan.
出版信息
Plant Physiol. 1992 Dec;100(4):1823-6. doi: 10.1104/pp.100.4.1823.
It is known that dormancy of the genus Dioscorea is induced by application of gibberellin (GA) A(3). To understand the role of GAs in dormancy induction, endogenous GAs have been identified by Kovats retention indices and full mass spectra from capillary gas chromatography-mass spectrometry analysis of purified extract from dormant bulbils of Dioscorea opposita Thunb. These include GA(4), GA(9), GA(12), GA(19), GA(20), GA(24), GA(36), and GA(53); their presence suggests the occurrence of two biosynthetic pathways in D. opposita bulbils, the early 13-hydroxylation pathway and the non-13-hydroxylation pathway.
已知薯蓣属植物的休眠是由施用赤霉素（GA）A3诱导的。为了解赤霉素在休眠诱导中的作用，通过气相色谱-质谱联用分析对来自山药休眠珠芽的纯化提取物进行分析，利用科瓦茨保留指数和全质谱鉴定了内源性赤霉素。这些赤霉素包括GA4、GA9、GA12、GA19、GA20、GA24、GA36和GA53；它们的存在表明山药珠芽中存在两条生物合成途径，即早期13-羟基化途径和非13-羟基化途径。
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