School of Forest Resources, North Carolina State University, Box 5488, Raleigh, North Carolina 27607.
Plant Physiol. 1968 Nov;43(11):1866-9. doi: 10.1104/pp.43.11.1866.
Some clones of Spirodela polyrrhiza form dormant bodies called turions which require several weeks of chilling treatment before they proceed to renew growth and develop into vegetative fronds. The individual fronds of Spirodela are less than 5 mm long and can be grown aseptically in liquid culture. Turion formation and germination can serve as a bioassay for the various compounds involved in dormancy development.Turion formation can be induced by manipulation of light intensity during the day, photoperiod, night temperature, day temperature, and concentration of nitrate in the culture medium. Different clones of Spirodela from northeastern United States, Puerto Rico, and Argentina had different requirements for turion formation. The clones from Argentina and Puerto Rico did not form turions under any of the experimental conditions imposed. Turions of some clones required chilling treatments for renewed vegetative growth while others did not. Both gibberellic acid and long photoperiods were required to bypass the chilling requirements of some clones, but not others.
有些轮藻属植物的克隆体形成休眠体，称为芽球，在它们重新开始生长并发育成营养叶之前，需要数周的冷藏处理。轮藻属的单个叶片长度不到 5 毫米，可以在无菌的液体培养基中生长。芽球的形成和萌发可以作为参与休眠发育的各种化合物的生物测定。通过白天光照强度、光周期、夜间温度、白天温度和培养基中硝酸盐浓度的控制，可以诱导芽球的形成。来自美国东北部、波多黎各和阿根廷的不同轮藻属克隆体对芽球形成有不同的要求。来自阿根廷和波多黎各的克隆体在任何实验条件下都不能形成芽球。一些克隆体的芽球需要冷藏处理才能重新进行营养生长，而另一些则不需要。赤霉素和长光周期都需要绕过一些克隆体的冷藏要求，但不是所有克隆体。
