Center for Phytotechnology, Rijksuniversiteit Leiden-Netherlands Organization for Applied Scientific Research, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
Plant Physiol. 1992 Dec;100(4):1834-9. doi: 10.1104/pp.100.4.1834.
The relationship between barley grain dormancy and gibberellic acid (GA(3)) responsiveness of aleurone layers has been investigated. Barley (Hordeum distichum L. cvs Triumph and Kristina) grains were matured under defined conditions in a phytotron. Grains of Triumph plants grown under long-day/warm conditions had lower dormancy levels than grains of plants grown under short-day/cool conditions. Aleurone layers isolated from grains of long-day Triumph plants secreted more alpha-amylase and had a higher responsiveness to GA(3) as measured by alpha-amylase secretion. Storage of the grains increased both the percentage of germination and the responsiveness of the aleurone to GA(3). Use of different sterilization methods to break dormancy confirmed the correlation between germination percentage and aleurone layer GA(3) responsiveness. The response of embryoless Triumph grains to GA(3) was lower than that of the isolated aleurone layers, suggesting a role of the starchy endosperm in regulating the GA(3) response of the aleurone layer. Grains of the cultivar Kristina harvested from short day- and long day-grown plants lacked dormancy, and their isolated aleurone layers had a similar responsiveness to GA(3) as measured by alpha-amylase secretion. The data indicate that the physiological state of the aleurone layers contributes to the percentage germination of the grains.
已研究大麦籽粒休眠与糊粉层赤霉素（GA（3））反应之间的关系。在植物生理培养箱中，根据明确的条件使大麦（Hordeum distichum L. cv. 特兰姆和克里斯蒂娜）成熟。在长日/温暖条件下生长的特兰姆植株的籽粒休眠水平低于在短日/凉爽条件下生长的植株的籽粒休眠水平。从长日特兰姆植株的籽粒中分离出来的糊粉层分泌更多的α-淀粉酶，并且如通过α-淀粉酶分泌所测量的，对 GA（3）的反应更高。籽粒的储存增加了发芽率和糊粉层对 GA（3）的反应性。使用不同的灭菌方法打破休眠证实了发芽率和糊粉层层 GA（3）反应性之间的相关性。无胚特兰姆籽粒对 GA（3）的反应性低于分离的糊粉层，这表明淀粉胚乳在调节糊粉层层对 GA（3）的反应中起作用。从短日和长日生长的植株收获的克里斯蒂娜品种的籽粒没有休眠，并且其分离的糊粉层层在 GA（3）反应性方面具有相似的α-淀粉酶分泌测量值。这些数据表明糊粉层层的生理状态有助于籽粒的发芽率。
