Vanlerberghe G C, McIntosh L
Michigan State University/Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824.
Plant Physiol. 1992 Dec;100(4):1846-51. doi: 10.1104/pp.100.4.1846.
In suspension cells of NT1 tobacco (Nicotiana tabacum L. cv bright yellow), inhibition of the cytochrome pathway of respiration with antimycin A induced a large increase in the capacity of the alternative pathway over a period of approximately 12 h, as confirmed in both whole cells and isolated mitochondria. The increase in alternative pathway capacity required de novo RNA and protein synthesis and correlated closely with the increase of a 35-kD alternative oxidase protein. When the cytochrome pathway of intact cells was inhibited by antimycin A, respiration proceeded exclusively through the alternative pathway, reached rates significantly higher than before antimycin A addition, and was not stimulated by p-trifluoromethoxycarbonylcyanide (FCCP). When inhibition of the cytochrome pathway was relieved, alternative pathway capacity and the level of the 35-kD alternative oxidase protein declined. Respiration rate also declined and could once again be stimulated by FCCP. These observations show that the capacities of the mitochondrial electron transport pathways can be regulated in a coordinate fashion.
在NT1烟草（Nicotiana tabacum L. cv bright yellow）的悬浮细胞中，用抗霉素A抑制细胞色素呼吸途径会在大约12小时内使交替途径的能力大幅增加，这在全细胞和分离的线粒体中均得到证实。交替途径能力的增加需要从头合成RNA和蛋白质，并且与一种35-kD交替氧化酶蛋白的增加密切相关。当完整细胞的细胞色素途径被抗霉素A抑制时，呼吸仅通过交替途径进行，达到的速率显著高于添加抗霉素A之前，并且不受对三氟甲氧基羰基氰化物（FCCP）的刺激。当细胞色素途径的抑制被解除时，交替途径能力和35-kD交替氧化酶蛋白水平下降。呼吸速率也下降，并且再次可以被FCCP刺激。这些观察结果表明，线粒体电子传递途径的能力可以以协调的方式进行调节。
