谷氨酰胺诱导离体玉米叶片组织中编码磷酸烯醇式丙酮酸羧化酶和碳酸酐酶的mRNA的氮依赖性积累。
Glutamine Induces the N-Dependent Accumulation of mRNAs Encoding Phosphoenolpyruvate Carboxylase and Carbonic Anhydrase in Detached Maize Leaf Tissue.
作者信息
Sugiharto B, Suzuki I, Burnell J N, Sugiyama T
机构信息
Department of Agricultural Chemistry, School of Agriculture, Nagoya University, Nagoya 464-01, Japan.
出版信息
Plant Physiol. 1992 Dec;100(4):2066-70. doi: 10.1104/pp.100.4.2066.
Abstract
We have used detached leaves to study the N-dependent control of expression of phosphoenolpyruvate carboxylase (PEPC) and carbonic anhydrase (CA) genes in maize (Zea mays L. cv Golden Cross Bantam T51). Following supplementation with an N-source and zeatin, PEPC and CA mRNA levels increased in leaves detached from N-deficient maize plants. Addition of methionine sulfoximine (MSX), a specific inhibitor of glutamine synthetase, inhibited the nitrate-dependent increase of PEPC and CA mRNA but did not affect the glutamine-dependent increase of PEPC and CA mRNA levels. Glutamine levels in detached maize leaves treated with various N sources in the presence or absence of MSX correlated with the levels of PEPC and CA mRNA. We conclude that glutamine is the most likely effector for controlling the N-dependent expression of PEPC and CA in maize plants.
摘要
我们利用离体叶片研究了氮素对玉米（Zea mays L. cv Golden Cross Bantam T51）中磷酸烯醇式丙酮酸羧化酶（PEPC）和碳酸酐酶（CA）基因表达的调控。在给缺氮玉米植株的离体叶片补充氮源和玉米素后，PEPC和CA的mRNA水平升高。添加谷氨酰胺合成酶的特异性抑制剂甲硫氨酸亚砜亚胺（MSX），可抑制硝酸盐依赖的PEPC和CA mRNA的增加，但不影响谷氨酰胺依赖的PEPC和CA mRNA水平的增加。在有或无MSX的情况下，用不同氮源处理的离体玉米叶片中的谷氨酰胺水平与PEPC和CA mRNA水平相关。我们得出结论，谷氨酰胺最有可能是控制玉米植株中PEPC和CA氮素依赖表达的效应物。
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