Ludwig S R, Oppenheimer D G, Silflow C D, Snustad D P
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5833-7. doi: 10.1073/pnas.84.16.5833.
The genome of Arabidopsis thaliana (Linnaeus) Heynhold was shown to contain an alpha-tubulin gene family consisting of at least four genes and/or pseudogenes. The primary structure of a transcribed alpha-tubulin gene was determined. A comparison of the predicted amino acid sequence of the A. thaliana alpha-tubulin with the predicted amino acid sequences of alpha-tubulins of Chlamydomonas reinhardtii, Stylonychia lemnae, and Homo spaiens reveals a high degree of homology; 90%, 87%, and 83% identity, respectively. Thus, a plant alpha-tubulin exhibits a high degree of homology to the alpha-tubulins of protists and animals. The coding sequence of the A. thaliana alpha-tubulin gene is interrupted by four introns, which occur at positions different from those of the less numerous introns of C. reinhardtii and rat alpha-tubulin genes. S1 nuclease mapping data showed that transcription is initiated 99 +/- 1 base pairs upstream from the translation initiation codon. Both 5' and 3' noncoding gene-specific probes were used to examine the expression of the alpha-tubulin gene in leaves, roots, and flowers by hybridization to total RNA isolated from these tissues. The results showed that the alpha-tubulin gene was transcribed in all three tissues.
拟南芥（林奈）海恩霍尔德的基因组被证明含有一个α-微管蛋白基因家族，该家族至少由四个基因和/或假基因组成。确定了一个转录的α-微管蛋白基因的一级结构。将拟南芥α-微管蛋白的预测氨基酸序列与莱茵衣藻、莱姆奈眼虫和智人的α-微管蛋白的预测氨基酸序列进行比较，发现具有高度同源性；分别为90%、87%和83%的同一性。因此，植物α-微管蛋白与原生生物和动物的α-微管蛋白表现出高度同源性。拟南芥α-微管蛋白基因的编码序列被四个内含子打断，这些内含子出现的位置与莱茵衣藻和大鼠α-微管蛋白基因中较少数量的内含子的位置不同。S1核酸酶图谱数据表明，转录起始于翻译起始密码子上游99±1个碱基对处。使用5'和3'非编码基因特异性探针，通过与从这些组织中分离的总RNA杂交，来检测α-微管蛋白基因在叶、根和花中的表达。结果表明，α-微管蛋白基因在所有这三种组织中都有转录。
