Huppe H C, Vanlerberghe G C, Turpin D H
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
Plant Physiol. 1992 Dec;100(4):2096-9. doi: 10.1104/pp.100.4.2096.
Addition of NO(3) (-) to N-limited Selenastrum minutum during photosynthesis resulted in an immediate drop in the NADPH/NADP ratio and a slower increase of the NADH/NAD ratio. These changes were accompanied by a rapid decrease in glucose-6-phosphate and increase in 6-phosphogluconate, indicating activation of glucose-6-phosphate dehydrogenase and a role for the oxidation pentose phosphate pathway during photosynthetic NO(3) (-) assimilation. In contrast, the short-term changes in pyridine nucleotides and metabolites during photosynthetic assimilation of NH(4) (+) were not consistent with a stimulation of the oxidative pentose phosphate pathway.
在光合作用期间向氮受限的微小新月菱形藻中添加硝酸根离子(NO₃⁻)会导致烟酰胺腺嘌呤二核苷酸磷酸(NADPH)/烟酰胺腺嘌呤二核苷酸磷酸(NADP)比值立即下降,以及烟酰胺腺嘌呤二核苷酸(NADH)/烟酰胺腺嘌呤二核苷酸(NAD)比值上升速度减慢。这些变化伴随着6-磷酸葡萄糖迅速减少和6-磷酸葡萄糖酸增加,表明6-磷酸葡萄糖脱氢酶被激活,且在光合硝酸根离子(NO₃⁻)同化过程中氧化戊糖磷酸途径发挥作用。相比之下,在光合铵离子(NH₄⁺)同化过程中,吡啶核苷酸和代谢物的短期变化与氧化戊糖磷酸途径的刺激不一致。