Cunningham L, Bowles N E, Archard L C
Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.
Br Med Bull. 1991 Oct;47(4):852-71. doi: 10.1093/oxfordjournals.bmb.a072516.
Nucleic acid was extracted from muscle biopsy samples from a series of highly selected patients suffering from chronic muscle fatiguability following a viral infection (Postviral Fatigue Syndrome: PVFS). Samples were examined for the presence of enteroviral RNA sequences or Epstein-Barr (EBV) virus DNA sequences by molecular hybridisation as these two agents have been implicated by retrospective serology in the aetiology of PVFS. We found enteroviral RNA in 24% of biopsy samples and EBV DNA in a further 9% of biopsy samples: no biopsy was positive for both enteroviral RNA and EBV DNA. In addition, in the case of enteroviruses we found that the persisting virus is defective in control of RNA replication as both strands of enteroviral RNA are present in similar amounts: this is unlike the asymmetric synthesis of genomic RNA seen in a productive, cytolytic enterovirus infection. The implications of these data in relation to mechanisms of viral persistence and muscle dysfunction are discussed.
从一系列经过严格挑选的、患有病毒感染后慢性肌肉疲劳（病毒性疲劳综合征：PVFS）的患者的肌肉活检样本中提取核酸。通过分子杂交检测样本中是否存在肠道病毒RNA序列或爱泼斯坦-巴尔（EBV）病毒DNA序列，因为这两种病原体已通过回顾性血清学研究被认为与PVFS的病因有关。我们在24%的活检样本中发现了肠道病毒RNA，在另外9%的活检样本中发现了EBV DNA：没有活检样本同时对肠道病毒RNA和EBV DNA呈阳性。此外，对于肠道病毒，我们发现持续存在的病毒在RNA复制控制方面存在缺陷，因为肠道病毒RNA的两条链含量相似：这与在有活性的、溶细胞性肠道病毒感染中所见的基因组RNA不对称合成不同。讨论了这些数据与病毒持续存在机制和肌肉功能障碍的关系。
