Leon-Monzon M, Dalakas M C
Neuromuscular Diseases Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Ann Neurol. 1992 Aug;32(2):219-22. doi: 10.1002/ana.410320215.
We searched for enteroviral nucleic acid sequences using the polymerase chain reaction and slot-blot hybridization in coded muscle biopsy specimens from 39 patients with active inflammatory myopathies (polymyositis, dermatomyositis, and inclusion-body myositis) and from 16 patients with other neuromuscular diseases, including patients with postpolio syndrome. For primers, we used sequences of the noncoding region at the 5' end of the viral RNA. We failed to detect specific enteroviral nucleic acid sequences in the muscle biopsy specimens. Because this sensitive technique can amplify even low copy numbers of the viral genome, it appears unlikely that a persistent enteroviral infection is the cause of inflammatory myopathies.
我们使用聚合酶链反应和狭缝印迹杂交技术，在39例活动性炎性肌病（多肌炎、皮肌炎和包涵体肌炎）患者以及16例其他神经肌肉疾病患者（包括脊髓灰质炎后综合征患者）的编码肌肉活检标本中，搜索肠道病毒核酸序列。对于引物，我们使用了病毒RNA 5'端非编码区的序列。我们未能在肌肉活检标本中检测到特定的肠道病毒核酸序列。由于这种敏感技术甚至可以扩增低拷贝数的病毒基因组，因此持续性肠道病毒感染似乎不太可能是炎性肌病的病因。
