Stenger A M, Garrè M L, Cama A, Andreussi L, Brisigotti M, Tonini G P, Cornaglia-Ferraris P
Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Genova, Italy.
Childs Nerv Syst. 1991 Nov;7(7):410-3. doi: 10.1007/BF00304209.
A sample of a primary brain tumor of glial cell origin was surgically removed from a 7-year-old girl. The histopathological analysis showed a heterogeneous tumor containing highly cellular areas composed of small, poorly differentiated cells with frequent mitoses suggestive of a glioblastoma multiforme. There were also areas presenting as features of lower-grade astrocytoma. Strong immunohistochemical staining for glial fibrillar acidic protein was demonstrated, while vimentin, neurofilament, and S-100 protein were all positive in just the astrocytic part of the tumor. The DNA extracted from a fresh tumor sample at diagnosis was processed by Southern blot analysis and hybridized with a 2.0 kb N-myc oncogene probe recognizing the first intron and the second exon of the human gene. A 20-fold amplification of the oncogene was found. The possible role of such a molecular alteration is discussed in light of the clinical presentation and histopathological features.
从一名7岁女孩身上手术切除了一个胶质细胞起源的原发性脑肿瘤样本。组织病理学分析显示，该肿瘤具有异质性，包含由小的、低分化细胞组成的高细胞区，有频繁的有丝分裂，提示多形性胶质母细胞瘤。也有呈现低级别星形细胞瘤特征的区域。胶质纤维酸性蛋白免疫组化染色呈强阳性，而波形蛋白、神经丝和S-100蛋白仅在肿瘤的星形细胞部分呈阳性。诊断时从新鲜肿瘤样本中提取的DNA通过Southern印迹分析进行处理，并与识别人类基因第一个内含子和第二个外显子的2.0 kb N-myc癌基因探针杂交。发现该癌基因有20倍的扩增。根据临床表现和组织病理学特征讨论了这种分子改变的可能作用。
