Moore T C, Anderson J D
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon.
Plant Physiol. 1966 Feb;41(2):238-43. doi: 10.1104/pp.41.2.238.
The effects of Tris-(2-diethylaminoethyl)-phosphate trihydrochloride (SK&F 7997-A(3)) on the development of 4 varieties of Pisum sativum were investigated. The compound inhibited shoot elongation of all 4 varieties by as much as 50% or more when seeds were soaked in solutions of the inhibitor for 12 hours before planting. Seed treatment also affected flowering by causing an increase in the number of nodes to the first flower in the early varieties Little Marvel and Alaska. The number of nodes preceding the first flower in the late varieties Dwarf and Tall Telephone was not affected by high concentrations of SK&F 7997-A(3), but low concentrations appeared to cause a slight reduction in the number of nodes to flower.The inhibitor had little effect on growth when applied to established seedlings; some slight inhibition was noted when high doses were applied to the shoot tip.SK&F 7997-A(3) suppressed the growth response of dwarf and tall peas to exogenous GA(3). The compound did not inhibit biosynthesis of gibberellin by Fusarium moniliformc when present in shaken liquid cultures at concentrations as high as 10 mg/ml. The inhibitor suppressed the action of applied GA(3) on shoot elongation when the 2 chemicals were applied in 3 ways: 1) inhibitor on lowermost compound leaf and GA(3) on shoot tip; 2) GA(3) on lowermost leaf and inhibitor on shoot tip; and 3) soaking of seeds in the 2 compounds combined for 12 hours prior to planting. The third method of dual treatment yielded evidence that SK&F 7997-A(3) interacts noncompetitively with GA(3) in the regulation of shoot elongation.
研究了磷酸三（2-二乙氨基乙基）酯三盐酸盐（SK&F 7997-A(3)）对4个豌豆品种生长发育的影响。在播种前将种子浸泡在抑制剂溶液中12小时，该化合物可抑制所有4个品种的茎伸长，抑制率高达50%或更高。种子处理还影响开花，使早熟品种小奇迹和阿拉斯加第一朵花出现之前的节数增加。晚熟品种矮生电话和高生电话第一朵花之前的节数不受高浓度SK&F 7997-A(3)的影响，但低浓度似乎会使开花前的节数略有减少。将抑制剂施用于已定植的幼苗时对生长影响不大；高剂量施用于茎尖时，有轻微抑制作用。SK&F 7997-A(3)抑制了矮生和高生豌豆对外源赤霉素（GA(3)）的生长反应。当该化合物以高达10 mg/ml的浓度存在于振荡液体培养物中时，它不会抑制串珠镰刀菌合成赤霉素。当以3种方式同时施用这两种化学物质时，该抑制剂抑制了施用的GA(3)对茎伸长的作用：1）抑制剂施用于最下部的复叶，GA(3)施用于茎尖；2）GA(3)施用于最下部的叶片，抑制剂施用于茎尖；3）在播种前将种子浸泡在两种化合物的混合液中12小时。第三种双重处理方法提供了证据，表明SK&F 7997-A(3)在调节茎伸长过程中与GA(3)发生非竞争性相互作用。
