Department of Biology, Wesleyan University, Middletown, Connecticut.
Plant Physiol. 1966 May;41(5):810-4. doi: 10.1104/pp.41.5.810.
Macroconidia of Fusarium solani f. phascoli have no detectable capacity to respire glucose anaerobically; germinated spores and mycelium, on the other hand, ferment glucose, although slowly.Extracts of ungerminated spores contain hexokinase, phosphohexoisomerase, phosphofructokinase, aldolase, triose phosphate dehydrogenase, triose phosphate isomerase, phosphoglyceric kinase, enolase, phosphoglyceric mutase, pyruvate kinase, and pyruvate decarboxylase. It follows, therefore, that the appearance of fermentative capacity during spore germination cannot be ascribed to the de novo synthesis of any of these enzymes.During germination and mycelial development the specific activity of all of the enzymes named except phosphohexoisomerase and aldolase increases 2- to 8-fold. Specific activity of all of the enzymes is substantially higher than the fermentative capacity of intact cells, i.e., none is limiting to anaerobic respiration.The enzymatic assay data are consistent with a conclusion reached earlier on the basis of studies of aerobic glucose metabolism, that the process of germination involves an acceleration of pre-existing metabolic systems rather than an appearance of new pathways.
尖孢镰刀菌茄腐变种的大型分生孢子在厌氧条件下几乎检测不到呼吸葡萄糖的能力；然而，发芽孢子和菌丝则可以缓慢地发酵葡萄糖。未发芽孢子的提取物中含有己糖激酶、磷酸己糖异构酶、磷酸果糖激酶、醛缩酶、三磷酸甘油醛脱氢酶、三磷酸甘油醛异构酶、磷酸甘油酸激酶、烯醇酶、磷酸甘油酸变位酶、丙酮酸激酶和丙酮酸脱羧酶。因此，在孢子发芽过程中出现发酵能力不能归因于这些酶的从头合成。在发芽和菌丝发育过程中，除磷酸己糖异构酶和醛缩酶外，所有命名酶的比活性增加了 2 到 8 倍。所有酶的比活性都明显高于完整细胞的发酵能力，即没有任何一种酶对无氧呼吸起限制作用。酶活性测定数据与先前基于有氧葡萄糖代谢研究得出的结论一致，即发芽过程涉及加速预先存在的代谢系统，而不是出现新途径。
