Department of Biology, Yale University, New Haven, Connecticut.
Plant Physiol. 1966 Nov;41(9):1485-8. doi: 10.1104/pp.41.9.1485.
Isatin, (indole 2,3-dione), which promotes elongation of Pisum stem sections at concentrations exceeding 0.1 mm, promotes elongation of Avena coleoptile sections only at higher concentrations, exceeding 1 mm. Aged isatin solutions are more active than fresh solutions, due to the slow, spontaneous conversion to isatate (o-aminophenylglyoxylate). A concentration of 0.1 mm aged isatin is as active in Avena coleoptile sections as in peas. Isatate has been independently synthesized and its auxin activity in both Avena coleoptile and Pisum stem sections confirmed. The synthetic isatate is more effective than isatin in both systems. This suggests that the auxin activity of isatin is due to its conversion to isatate.
靛红(吲哚-2,3-二酮),在浓度超过 0.1 毫米时能促进豌豆茎切段伸长,仅在浓度超过 1 毫米时才促进燕麦胚芽鞘切段伸长。由于靛红缓慢自发转化为异羟肟酸(邻氨基苯甘氨酸),老化的靛红溶液比新鲜溶液更具活性。0.1 毫米浓度的老化靛红在燕麦胚芽鞘切段中的活性与豌豆中相同。已独立合成异羟肟酸,并证实其在燕麦胚芽鞘和豌豆茎切段中的生长素活性。在这两个系统中,合成的异羟肟酸比靛红更有效。这表明,靛红的生长素活性是由于其转化为异羟肟酸。