Cleland R
Plant Physiol. 1967 Feb;42(2):271-4. doi: 10.1104/pp.42.2.271.
A study has been made of the hydroxyproline-induced inhibition of elongation of Avena coleoptile tissues. The isomers of 4-hydroxyproline differ in their effectiveness; only the L isomers are growth inhibitors with the cis form (allohydroxyproline) being more effective than the trans form (hydroxyproline). Hydroxyproline differs from other amino acid antagonists and protein synthesis inhibitors in respect to 2 characteristics of the growth inhibition. First, a certain increment of auxin-induced elongation must take place following addition of hydroxyproline before the growth is inhibited. In contrast, pretreatment with other amino acid antagonists or protein synthesis inhibitors completely eliminates the ability of Avena coleoptile sections to respond to auxin. Secondly, sucrose markedly increases the magnitude of the hydroxyproline inhibition; i.e., sucrose acts to inhibit rather than promote growth when in the presence of hydroxyproline. It appears that hydroxyproline is a specific inhibitor for the synthesis of some factor which is utilized in elongation. Following addition of hydroxyproline, auxin-induced elongation continues until the pool of this factor is exhausted; then elongation is inhibited.
对羟基脯氨酸诱导的燕麦胚芽鞘组织伸长抑制进行了一项研究。4-羟基脯氨酸的异构体在其效力上有所不同；只有L异构体是生长抑制剂，其中顺式异构体（别羟基脯氨酸）比反式异构体（羟基脯氨酸）更有效。羟基脯氨酸在生长抑制的两个特征方面不同于其他氨基酸拮抗剂和蛋白质合成抑制剂。首先，在生长受到抑制之前，添加羟基脯氨酸后，生长素诱导的伸长必须有一定程度的增加。相比之下，用其他氨基酸拮抗剂或蛋白质合成抑制剂预处理会完全消除燕麦胚芽鞘切段对生长素作出反应的能力。其次，蔗糖显著增加了羟基脯氨酸抑制的程度；也就是说，在存在羟基脯氨酸的情况下，蔗糖起到抑制而非促进生长的作用。似乎羟基脯氨酸是用于伸长过程中某种因子合成的特异性抑制剂。添加羟基脯氨酸后，生长素诱导的伸长会持续，直到该因子的储备耗尽；然后伸长受到抑制。
