State University of New York, Buffalo, New York.
Plant Physiol. 1966 Nov;41(9):1489-98. doi: 10.1104/pp.41.9.1489.
Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded carbohydrate-rich fractions with solubility characteristics ranging from pectic substance to hemicellulose yet the uronic acid and pentose composition of these fractions was decidedly pectic. Galacturonic acid was the only uronic acid present in each fraction. Subfractionation of alkali-soluble (hemicellulosic) polysaccharide by neutralization followed by ethanol precipitation gave 3 fractions, a water-insoluble, an ethanol-insoluble, and an ethanol-soluble fraction, each progressively poorer in galacturonic acid units and progressively richer in arabinose units; all relatively poor in xylose units.Apparently, processes involved in biosynthesis of primary cell wall continued to produce pectic substance during cell enlargement while processes leading to biosynthesis of typically secondary cell wall polysaccharide such as 4-0-methyl glucuronoxylan were not activated.
在含有肌醇-2-(3)H 的琼脂培养基上长时间培养悬铃木（Acer pseudoplatanus L.）细胞培养物，导致标签主要掺入细胞壁多糖的糖醛酸基和戊糖基单元。通常用于区分果胶物质和半纤维素的程序产生了富含碳水化合物的馏分，其溶解度特征从果胶物质到半纤维素，但这些馏分的戊糖酸和戊糖组成明显是果胶物质。每个馏分中仅存在半乳糖醛酸。用中和随后用乙醇沉淀对碱溶性（半纤维素）多糖进行亚组分分离，得到 3 个馏分，即不溶于水的、不溶于乙醇的和溶于乙醇的馏分，每个馏分的半乳糖醛酸单元逐渐减少，阿拉伯糖单元逐渐增加；所有这些都相对缺乏木糖单元。显然，在细胞增大过程中，参与初生细胞壁生物合成的过程继续产生果胶物质，而导致典型次生细胞壁多糖（如 4-O-甲基葡萄糖醛酸木聚糖）生物合成的过程并未被激活。
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