Department of Botany, University of Illinois, 61801, Urbana, IL, USA.
Planta. 1978 Jan;141(1):77-82. doi: 10.1007/BF00387748.
Tobacco (Nicotiana tabacum L.) cells were cultured in a liquid medium which contained sucrose as a source of carbon and energy. Various cell-wall constituents and wall precursors (L-arabinose, D-xylose, D-galactose, D-mannose, D-glucuronate, myo-inositol) were added to cells growing in this medium to by-pass possible rate-limiting steps in the relevant metabolic pathways. None of these compounds stimulated growth as measured by increase in fresh weight; myo-inositol did cause a slight increase and L-arabinose a decrease in dry weight accumulation compared to controls grown on sucrose only. Although myo-inositol was not needed for rapid growth, tracer level amounts of [2-(3)H]myo-inositol were rapidly absorbed and metabolized. Label was incorporated into the uronide and pentose residues of cell walls and exocellular polysaccharide.
烟草（Nicotiana tabacum L.）细胞在含有蔗糖作为碳源和能源的液体培养基中培养。将各种细胞壁成分和细胞壁前体（L-阿拉伯糖、D-木糖、D-半乳糖、D-甘露糖、D-葡萄糖醛酸、肌醇）添加到在该培养基中生长的细胞中，以绕过相关代谢途径中可能的限速步骤。这些化合物都没有像通过仅用蔗糖培养的对照细胞那样，通过增加鲜重来刺激生长；与对照细胞相比，肌醇确实略微增加了，而 L-阿拉伯糖则减少了干重的积累。尽管肌醇不是快速生长所必需的，但示踪水平的[2-（3）H]肌醇被迅速吸收和代谢。标记被掺入细胞壁和细胞外多糖的糖醛酸和戊糖残基中。
