Tuli V, Moyed H S
Department of Microbiology, University of Southern California, School of Medicine, Los Angeles, California 90033.
Plant Physiol. 1967 Mar;42(3):425-30. doi: 10.1104/pp.42.3.425.
Extracts of pea seedlings (Pisum sativum, variety Alaska) oxidize indole-3-acetic acid to a bacteriostatic compound which has been identified as 3-hydroxymethyloxindole. At physiological pH this compound is readily dehydrated to 3-methyleneoxindole, another bacteriostatic agent. The extracts of pea seedlings also contain a reduced triphosphopyridine nucleotide-linked enzyme which reduces 3-methyleneoxindole to 3-methyloxindole, a non-toxic compound.These enzymatic reactions also take place in intact seedlings; thus, a pathway of indole-3-acetic acid degradation via oxindoles appears to be pertinent to plant metabolism.The significance of such metabolism lies in the fact that a key intermediate of this pathway, 3-methyleneoxindole, is a sulfhydryl reagent capable of profound effects on metabolism and growth.
豌豆幼苗(豌豆,阿拉斯加品种)提取物可将吲哚 - 3 - 乙酸氧化为一种抑菌化合物,该化合物已被鉴定为3 - 羟甲基吲哚酮。在生理pH值下,这种化合物很容易脱水形成另一种抑菌剂3 - 亚甲基吲哚酮。豌豆幼苗提取物还含有一种与还原型三磷酸吡啶核苷酸相关的酶,该酶可将3 - 亚甲基吲哚酮还原为无毒化合物3 - 甲基吲哚酮。这些酶促反应也发生在完整的幼苗中;因此,通过吲哚酮进行吲哚 - 3 - 乙酸降解的途径似乎与植物代谢相关。这种代谢的意义在于,该途径的关键中间体3 - 亚甲基吲哚酮是一种巯基试剂,能够对代谢和生长产生深远影响。