Departments of Biology, Entomology and Pesticide Research Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802.
Plant Physiol. 1976 Jul;58(1):77-81. doi: 10.1104/pp.58.1.77.
An indoleacetic acid oxidase preparation from an acetone powder of Parthenocissus tricuspidata crown-gall tissue has been examined. An intermediate in the reaction is 3-hydroxymethyloxindole and nonenzymic conversion of it to 3-methyleneoxindole was observed. Neither reaction mixtures nor 3-methyleneoxindole have any auxin-like activity in Avena or wheat coleoptile bioassays. In vivo studies show that although 53% decarboxylation of indoleacetic acid was observed in 48 hours, only a small amount of 3-methyloxindole could be recovered from the medium. The other decarboxylated products remain to be identified but are not 3-hydroxymethyloxindole or 3-methyleneoxindole.
从三叶木通冠瘿组织的丙酮粉中提取的吲哚乙酸氧化酶制剂已经过检查。反应的中间产物是 3-羟甲基氧吲哚,并且观察到它非酶促转化为 3-亚甲基氧吲哚。在燕麦或小麦胚芽鞘生物测定中,无论是反应混合物还是 3-亚甲基氧吲哚都没有任何类似生长素的活性。体内研究表明,尽管在 48 小时内观察到吲哚乙酸的 53%脱羧,但从中性介质中只能回收少量的 3-甲氧基氧吲哚。其他脱羧产物仍有待鉴定,但不是 3-羟甲基氧吲哚或 3-亚甲基氧吲哚。