Taylor A O, Bonner B A
Department of Botany, University of California, Davis, California 95616.
Plant Physiol. 1967 Jun;42(6):762-6. doi: 10.1104/pp.42.6.762.
Phytochrome has been isolated from the green alga Mesotaenium and the liverwort Sphaerocarpos. The Mesotaenium pigment had absorption peaks at 649 and 710 nm for the P(R) and P(FR) forms, respectively. Corresponding difference spectrum maxima for the Sphaerocarpos pigment were at 655 and 720 nm. While the absorption maxima differ, the reversibility and efficiency with which red and far-red light transform the Mesotaenium pigment are very similar to that reported for phytochrome isolated from etiolated seedlings of higher plants. Methods are described which allow efficient separation of phytochrome from highly pigmented light-grown material.
已经从绿藻微星鼓藻和地钱中分离出了光敏色素。微星鼓藻色素的P(R)和P(FR)形式分别在649和710纳米处有吸收峰。地钱色素相应的差示光谱最大值分别在655和720纳米处。虽然吸收最大值不同,但红光和远红光转化微星鼓藻色素的可逆性和效率与从高等植物黄化幼苗中分离出的光敏色素非常相似。文中描述了从色素含量高的光照生长材料中有效分离光敏色素的方法。