Gassman M, Bogorad L
Department of Botany, University of Chicago, Chicago, Illinois 60637.
Plant Physiol. 1967 Jun;42(6):781-4. doi: 10.1104/pp.42.6.781.
The effects of various inhibitors of nucleic acid and protein synthesis on protochlorophyllide synthesis in dark-grown Phaseolus vulgaris var. Red Kidney have been studied. Actinomycin D, chloramphenicol, and puromycin inhibit the regeneration of protochlorophyllide holochrome (detected as a 650 mmu absorption peak) in vivo in darkness after photoconversion of endogenous protochlorophyllide a to chlorophyllide a; this inhibition does not occur in similarly treated leaves supplied with delta-aminolevulinic acid.These data suggest that the regeneration of protochlorophyllide results from the synthesis of RNA and enzymes required for the production of delta-aminolevulinate.
研究了各种核酸和蛋白质合成抑制剂对黑暗中生长的菜豆变种红腰豆中原叶绿素酸酯合成的影响。放线菌素D、氯霉素和嘌呤霉素抑制了内源性原叶绿素酸酯a光转化为叶绿素酸酯a后,黑暗中体内原叶绿素酸酯全色素(检测为650毫微米吸收峰)的再生;在供应δ-氨基乙酰丙酸的类似处理叶片中,这种抑制作用不会发生。这些数据表明,原叶绿素酸酯的再生是由δ-氨基乙酰丙酸产生所需的RNA和酶的合成导致的。