可光转化和非可光转化原叶绿素之间的关系。
Relationship between Photoconvertible and Nonphotoconvertible Protochlorophyllides.
机构信息
Department of Biology, Brandeis University, Waltham, Massachusetts 02154.
出版信息
Plant Physiol. 1971 Oct;48(4):383-8. doi: 10.1104/pp.48.4.383.
Two forms of protochlorophyllide are found in dark-grown bean (Phaseolus vulgaris, var. Black Velentine) leaves, one (protochlorophyllide(650)) which is directly photoconvertible to chlorophyllide and another (protochlorophyllide(632)) which is not. Dark-grown leaves placed in solutions of delta-aminolevulinic acid accumulate protochlorophyllide(632). Protochlorophyllide(650) and protochlorophyllide(632) can be partially separated on sucrose density gradients. A nitrogen atmosphere blocks chlorophyll synthesis in light or the regeneration of protochlorophyllide(650) in the dark, even in the presence of excess delta-aminolevulinic acid, except when a stockpile of protochlorophyllide(632) is present in the leaf. Under the latter conditions chlorophyll synthesis or protochlorophyllide(650) regeneration is accompanied by a decrease in protochlorophyllide(632). These experiments suggest that protochlorophyllide(632) may be converted to protochlorophyllide(650).Cycloheximide inhibited greening only after an "action-dependent" delay, requiring a predictable minimal period of illumination. This inhibition could be relieved for a time by feeding delta-aminolevulinic acid.
在黑暗中生长的菜豆（Phaseolus vulgaris，var. Black Valentine）叶片中存在两种原叶绿素，一种（原叶绿素（650））可直接光转化为叶绿素，另一种（原叶绿素（632））则不能。在 δ-氨基酮戊酸溶液中培养的黑暗生长的叶片会积累原叶绿素（632）。原叶绿素（650）和原叶绿素（632）可在蔗糖密度梯度上部分分离。在氮气氛中，即使存在过量的 δ-氨基酮戊酸，光照也会阻止叶绿素的合成或黑暗中原叶绿素（650）的再生，除非叶片中有原叶绿素（632）的储备。在后一种情况下，叶绿素合成或原叶绿素（650）的再生伴随着原叶绿素（632）的减少。这些实验表明，原叶绿素（632）可能转化为原叶绿素（650）。环己酰亚胺仅在“依赖作用”的延迟后才抑制变绿，需要可预测的最小光照时间。这种抑制可以通过喂食 δ-氨基酮戊酸暂时缓解一段时间。
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