Kuźmak J, Moussa A, Grundboeck-Juśko J
Department of Biochemistry, Institute of Veterinary Sciences, Puławy, Poland.
Acta Biochim Pol. 1991;38(1):107-10.
The polymerase-chain reaction was applied for detection of provirus DNA of the bovine leukaemia virus (BLV). A short fragment of 292 bp including region R and U5 LTR 5' of BLV was amplified, and the optimum parameters of amplification of this fragment were established. Electrophoresis revealed the presence of the 292 bp fragment from the leucocytes of four out of six cows showing a positive serological response to BLV antigens. Application of the polymerase-chain reaction in diagnosis of bovine leukaemia is suggested.
应用聚合酶链反应检测牛白血病病毒（BLV）的前病毒DNA。扩增出了一段包含BLV的R区和U5 LTR 5'的292 bp短片段，并确定了该片段扩增的最佳参数。电泳显示，在对BLV抗原呈血清学阳性反应的6头奶牛中，有4头奶牛的白细胞存在292 bp片段。建议将聚合酶链反应应用于牛白血病的诊断。
