Teichler-Zallen D, Levine R P
The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Plant Physiol. 1967 Nov;42(11):1643-7. doi: 10.1104/pp.42.11.1643.
Changes in the intensity of the electron spin resonance signal of divalent manganese were found to occur in suspensions of wild-type Chlamydomonas reinhardi. The observed manganese signal decreased in the light and increased in the dark. Through the use of a continuous-flow system it was possible to determine that the manganous ions responsible for the observed signal were localized solely in the medium. Changes in the signal intensity associated with wild-type cells were independent of the ability of fragments prepared from these cells to perform the Hill reaction with 2,6-dichlorophenol-indophenol (DPIP) as the oxidant.The manganese signal changes were still evident, though smaller, in cell suspensions of wild-type cells treated with 3-(3,4-dichlorophenyl)-1, 1-dimethylurea, and in mutant strains unable to carry out the Hill reaction, ac-115 and ac-141.From these data it is concluded that the changes in intensity of the manganese resonance are not related to the function of manganese in photosynthesis but may reflect the capacity of cells for ion uptake in the light.
在野生型莱茵衣藻的悬浮液中发现了二价锰电子自旋共振信号强度的变化。观察到的锰信号在光照下降低，在黑暗中增加。通过使用连续流动系统，可以确定产生观察到的信号的锰离子仅存在于培养基中。与野生型细胞相关的信号强度变化与从这些细胞制备的片段以2,6-二氯酚靛酚（DPIP）作为氧化剂进行希尔反应的能力无关。在用3-(3,4-二氯苯基)-1,1-二甲基脲处理的野生型细胞的细胞悬浮液中，以及在无法进行希尔反应的突变菌株ac-115和ac-141中，锰信号变化仍然明显，尽管较小。从这些数据可以得出结论，锰共振强度的变化与锰在光合作用中的功能无关，但可能反映了细胞在光照下吸收离子的能力。
