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光和化学生长调节剂对菜豆叶片衰老的调节。

Regulation of senescence in bean leaf discs by light and chemical growth regulators.

机构信息

Department of Botany, University of California, Berkeley, California 94720.

出版信息

Plant Physiol. 1967 Dec;42(12):1757-62. doi: 10.1104/pp.42.12.1757.

DOI:10.1104/pp.42.12.1757
PMID:16656716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1086793/
Abstract

The senescence of excised discs of primary leaves of Phaseolus vulgaris, L., var. Red Kidney was followed by measuring the net breakdown of protein and chlorophyll. The chemical growth regulators indoleacetic acid, 2,4-dichlorophenoxy-acetic acid, gibberellic acid, kinetin, and 6-benzylaminopurine were relatively ineffective in retarding senescence in this tissue. White light, on the other hand, was very effective in senescence retardation. The response to light did not have the characteristics of a low energy (phytochrome) response and was blocked by concentrations of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea which inhibited photosynthesis in the leaf discs. The light-induced retardation of senescence was concluded to be dependent on photosynthesis.

摘要

离体菜豆(Phaseolus vulgaris,L.,var. Red Kidney)初生叶片的衰老可以通过测量蛋白质和叶绿素的净降解来跟踪。化学生长调节剂吲哚乙酸、2,4-二氯苯氧基乙酸、赤霉素、激动素和 6-苄基氨基嘌呤在延缓该组织衰老方面效果相对较差。另一方面,白光在延缓衰老方面非常有效。这种对光的反应不具有低能量(光敏素)反应的特征,并且可以被 3-(3,4-二氯苯基)-1,1-二甲基脲的浓度阻断,该浓度抑制叶片圆片的光合作用。可以得出结论,光诱导的衰老延缓依赖于光合作用。

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Regulation of senescence in bean leaf discs by light and chemical growth regulators.光和化学生长调节剂对菜豆叶片衰老的调节。
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本文引用的文献

1
Effect of Kinetin on Protein & Nucleic Acid Metabolism in Xanthium Leaves During Senescence.激动素对衰老过程中苍耳叶片蛋白质和核酸代谢的影响。
Plant Physiol. 1962 Sep;37(5):595-602. doi: 10.1104/pp.37.5.595.
2
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol.叶绿素a和叶绿素b及其脱镁叶绿素在乙醇中的分光光度特性。
Biochim Biophys Acta. 1965 Nov 29;109(2):448-53. doi: 10.1016/0926-6585(65)90170-6.