琥珀酸氧化在番茄果实线粒体中的特性。
Properties of succinate oxidation in tomato fruit mitochondria.
机构信息
Vegetable Crops Building, University of Illinois, Urbana, Illinois 61801.
出版信息
Plant Physiol. 1968 Feb;43(2):248-54. doi: 10.1104/pp.43.2.248.
Mitochondria from tomato fruit (Lycopersicon esculentum Mill.) exhibited a respiratory control ratio of 2.5 and an ADP:O ratio of 1.3 for succinate oxidation for 24 hours after isolation. They also showed a delay in response to the first addition of ADP. The addition of ATP and ADP before succinate eliminated the delayed response as did chelation of endogenous cations with ethylenediaminetetraacetic acid. The addition of ATP after succinate resulted in a longer delay in response than that obtained with ADP. Exogenous oxaloacetate in low concentration inhibited respiration in states 3 and 4 with succinate and resulted in delayed response to ADP. The function of adenine nucleotide during the delay in response may be to promote the metabolism of oxaloacetate or to decrease the affinity of oxaloacetate to its site of inhibition.
番茄果实（Lycopersicon esculentum Mill.）的线粒体在分离后 24 小时内，对琥珀酸氧化的呼吸控制比为 2.5，ADP:O 比为 1.3。它们对 ADP 的第一次添加也表现出延迟反应。在添加琥珀酸之前添加 ATP 和 ADP，以及用乙二胺四乙酸螯合内源性阳离子，都可以消除这种延迟反应。在添加琥珀酸之后再添加 ATP，会导致比添加 ADP 更长的延迟反应。低浓度的外源草酰乙酸抑制琥珀酸状态 3 和 4 的呼吸，并导致对 ADP 的延迟反应。在延迟反应期间，腺嘌呤核苷酸的作用可能是促进草酰乙酸的代谢，或降低草酰乙酸与其抑制部位的亲和力。
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