Department of Horticulture, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1970 Aug;46(2):200-3. doi: 10.1104/pp.46.2.200.
Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria could be stored successfully in the frozen state without a cryoprotective agent if the mitochondria were frozen quickly by immersion in liquid nitrogen and later thawed quickly at 30 C. Criteria of freezing damage were rate of respiration, adenosine diphosphate to oxygen ratio, and respiratory control ratio. Marked reduction in respiration and loss of respiratory control occurred when mitochondria were transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 C. Dimethylsulfoxide (5%) prevented freezing damage when mitochondria were incubated at -5 C but did not prevent freezing damage at -10 or -18 C. Isolated tomato mitochondria show promise as a model system for studying the nature of freezing damage and the mode of action of cryo-protective agents.
如果将番茄（Lycopersicon esculentum var. Kc 146）果实线粒体迅速浸入液氮中进行冷冻，而无需添加冷冻保护剂，那么这些线粒体就可以成功地在冷冻状态下储存。冷冻损伤的标准为呼吸速率、腺苷二磷酸与氧的比值和呼吸控制比。如果将线粒体从液氮中转移到-5℃、-10℃或-18℃并在 30℃下解冻之前先在这些温度下放置 15 分钟，那么线粒体的呼吸作用会明显减弱，呼吸控制也会丧失。当线粒体在-5℃下孵育时，二甲基亚砜（5%）可以防止冷冻损伤，但在-10℃或-18℃时不能防止冷冻损伤。分离的番茄线粒体有望成为研究冷冻损伤性质和冷冻保护剂作用模式的模型系统。
