有籽和无籽托凯葡萄果实中的类赤霉素活性
Gibberellin-like Activity in Berries of Seeded and Seedless Tokay Grapes.
作者信息
Iwahori S, Weaver R J, Pool R M
机构信息
Department of Viticulture and Enology, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1968 Mar;43(3):333-7. doi: 10.1104/pp.43.3.333.
Gibberellin-like activity in berries of both ;Tokay' (seeded) and ;Seedless Tokay' grapes (Vitis vinifera L.) at different stages of development was determined. Activity in both seeded and seedless berries was mainly in the acidic ethyl acetate fraction. In seeded berries there was very high activity beginning at the early fruit-set stage and persisting for about 3 weeks after which the activity fell to low level and vanished by the middle of July. On August 1 there was another activity peak. The activity in ;Seedless Tokay' was similar except the decline was considerably more rapid and a secondary peak was reached in mid-June instead of August. The gibberellin-like substances in the acidic ethyl acetate fraction occurred mainly between R(F) 0.3 and 0.6 when the chromatogram was developed with ammoniacal isopropanol.The gibberellin-like substances from both types of berries were active in dwarf pea, dwarf corn, and lettuce hypocotyl bioassays, but not in the cucumber hypocotyl test. The role of gibberellin-like substances in berry development is discussed.
测定了“瑰宝”（有籽）和“无核瑰宝”葡萄（欧亚种葡萄）果实发育不同阶段浆果中的类赤霉素活性。有籽和无核浆果中的活性主要存在于酸性乙酸乙酯组分中。在有籽浆果中，从坐果初期开始就有非常高的活性，并持续约3周，之后活性降至低水平，并在7月中旬消失。8月1日出现另一个活性高峰。“无核瑰宝”中的活性情况类似，只是下降速度更快，并且在6月中旬而非8月达到第二个高峰。当用氨性异丙醇展开色谱图时，酸性乙酸乙酯组分中的类赤霉素物质主要出现在比移值（R(F)）0.3至0.6之间。两种浆果中的类赤霉素物质在矮生豌豆、矮生玉米和生菜下胚轴生物测定中具有活性，但在黄瓜下胚轴试验中无活性。文中讨论了类赤霉素物质在浆果发育中的作用。
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