Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing, PR China.
Proteomics. 2012 Jan;12(1):86-94. doi: 10.1002/pmic.201000668. Epub 2011 Dec 2.
Gibberellin (GA) is widely used in the table grape and raisin industries to enlarge the berries of seedless varieties. However, the mechanism underlying its berry-sizing effect is poorly understood. In this study, clusters of Centennial Seedless (Vitis vinifera L.) were treated with 30 ppm GA3 on day 12 after flowering, and berries were sampled at development stages I, II and III for proteomic analysis. Among the 1479 proteins detected on 2-DE maps, 19, 70 and 69 spots in stages I, II and III, respectively, showed an at least twofold difference in volume between treatments and controls. Of these, 125 proteins were successfully identified and assigned to eight functional groups, chief among them are metabolism and energy, stress response, expression regulation and cytoskeleton proteins. Stress-response proteins were predominantly down-regulated in GA3-treated berries in stages I and II, and significantly up-regulated in stage III. Up-regulation of cytoskeleton, cell-wall modification and other important proteins was found in the two latter stages of berry development. Our proteomic results and subsequent validation revealed, for the first time, the role of redox homeostasis in GA3-induced berry enlargement and markedly remodeled cellular protein expression in treated berries.
赤霉素（GA）广泛应用于酿酒葡萄和葡萄干产业，以增大无核品种的浆果。然而，其浆果增大作用的机制尚未完全了解。本研究中，在开花后第 12 天用 30ppm GA3 处理 Centennial Seedless（Vitis vinifera L.）果穗，并在发育阶段 I、II 和 III 时取样进行蛋白质组学分析。在 2-DE 图谱上检测到的 1479 种蛋白质中，分别有 19、70 和 69 个斑点在 I、II 和 III 阶段的体积与对照组相比至少有两倍差异。其中，125 种蛋白质被成功鉴定，并被分配到八个功能组，其中主要包括代谢和能量、应激反应、表达调控和细胞骨架蛋白。在 GA3 处理的浆果的 I 和 II 阶段，应激反应蛋白主要下调，而在 III 阶段则显著上调。在浆果发育的后两个阶段，细胞骨架、细胞壁修饰和其他重要蛋白的上调。我们的蛋白质组学结果和随后的验证首次揭示了氧化还原平衡在 GA3 诱导的浆果增大中的作用，以及处理过的浆果中细胞蛋白表达的显著重塑。
