Department of Agronomy, University of Kentucky, Lexington, Kentucky 40506.
Plant Physiol. 1968 Apr;43(4):484-8. doi: 10.1104/pp.43.4.484.
Alcohols and hydrogen peroxide altered the permeability of membranes of Beta vulgaris root cells. Generally alcohols increased the permeability of membranes without going through an induction period except methanol which required a 10- to 15-hour induction period. The membrane effect of methanol could be inhibited with CaCl(2), cholesterol, beta-sitosterol, and stigmasterol. Cholesterol was the most effective inhibitor, followed by beta-sitosterol and stigmasterol; and at the same concentration, the sterols were more effective than CaCl(2), the classic membrane stabilizer.Ergosterol increased the methanol-initiated betacyanin leakage. Since none of the tested sterols reversed the betacyanin efflux induced by hydrogen peroxide, the sterols do not apparently act as antioxidants. The results are explained in terms of sterol-phospholipid interaction, based on stereochemistry and charge distribution.
醇和过氧化氢改变了菠菜根细胞的膜通透性。一般来说，醇类会增加膜的通透性，而不需要经过诱导期，除了甲醇，甲醇需要 10 到 15 小时的诱导期。甲醇的膜效应可以用氯化钙、胆固醇、β-谷甾醇和豆甾醇来抑制。胆固醇是最有效的抑制剂，其次是β-谷甾醇和豆甾醇；而且在相同的浓度下，固醇比经典的膜稳定剂氯化钙更有效。麦角固醇增加了甲醇引发的甜菜红素渗漏。由于测试的固醇中没有一种能逆转由过氧化氢引起的甜菜红素外排，因此固醇显然不作为抗氧化剂起作用。根据立体化学和电荷分布，这些结果可以用固醇-磷脂相互作用来解释。
