Department of Botany, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1968 May;43(5):811-4. doi: 10.1104/pp.43.5.811.
Respiration-linked contraction of corn mitochondria is not noticeably reduced by low, uncoupling concentrations of dinitrophenol. However, if a contraction/respiration ratio is calculated, the contraction proves to be uncoupled. Previous statements that contraction cannot be uncoupled from respiration are in error.The uncoupling of contraction is consistent with the concept that dinitrophenol attacks a primary non-phosphorylated high energy intermediate (I approximately X). It is proposed that this intermediate is linked to some contractile mechanism such that the degree of contraction reflects the level of intermediate.
呼吸相关联的玉米线粒体收缩,不会因二硝基酚的低浓度而明显减少,这种浓度下不会发生解偶联。但是,如果计算收缩/呼吸比值,就会发现收缩发生了解偶联。之前关于收缩不能与呼吸解偶联的说法是错误的。收缩的解偶联与二硝基酚攻击一个主要的非磷酸化高能中间物(I 近似于 X)的概念是一致的。据推测,这个中间物与某种收缩机制相联系,收缩的程度反映了中间物的水平。