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4,5,6,7-四氯-2-三氟甲基苯并咪唑对呼吸链磷酸化的解偶联作用。

The uncoupling of respiratory-chain phosphorylation by 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole.

作者信息

Beechey R B

出版信息

Biochem J. 1966 Jan;98(1):284-9. doi: 10.1042/bj0980284.

DOI:10.1042/bj0980284
PMID:4223043
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1264827/
Abstract
  1. 30mmum-4,5,6,7-Tetrachloro-2-trifluoromethylbenzimidazole (TTFB) uncouples respiratory-chain phosphorylation. The respiration rate of uncoupled mitochondria is significantly greater than the state 3 rate. Neither added orthophosphate nor ATP is required to sustain this rate. The oligomycin- and octylguanidine-induced inhibitions of respiration are relieved by uncoupling concentrations of TTFB. 2. Uncoupling concentrations of TTFB elicit a high adenosinetriphosphatase activity and inhibit the ATP-dependent succinate-linked reduction of NAD catalysed by submitochondrial particles from ox-heart mitochondria. 3. The action of TTFB is similar to that of 2,4-dinitrophenol. Evidence is presented which shows that it is the anionic forms of benzimidazoles and imidazoles, having the negative charge localized on the ring nitrogen atom, which are the effective uncoupling agents.
摘要
  1. 30毫米的4,5,6,7-四氯-2-三氟甲基苯并咪唑（TTFB）可使呼吸链磷酸化解偶联。解偶联线粒体的呼吸速率显著高于状态3速率。维持该速率既不需要添加正磷酸盐也不需要ATP。解偶联浓度的TTFB可解除寡霉素和辛基胍对呼吸的抑制作用。2. 解偶联浓度的TTFB会引发高腺苷三磷酸酶活性，并抑制由牛心线粒体亚线粒体颗粒催化的ATP依赖性琥珀酸连接的NAD还原反应。3. TTFB的作用与2,4-二硝基苯酚相似。有证据表明，正是苯并咪唑和咪唑的阴离子形式，其负电荷定位于环氮原子上，才是有效的解偶联剂。

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1
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Biochem J. 1966 Jan;98(1):284-9. doi: 10.1042/bj0980284.
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本文引用的文献

1
A METHOD FOR THE COLORIMETRIC DETERMINATION OF PHOSPHORUS.磷的比色测定法。
Science. 1944 Nov 3;100(2601):413-4. doi: 10.1126/science.100.2601.413.
2
The effect of 5,6-dimethylbenzimidazole and related compounds on the growth of Lactobacillus lactis Dorner.5,6-二甲基苯并咪唑及相关化合物对乳酸乳球菌多纳变种生长的影响。
J Bacteriol. 1951 Nov;62(5):633-8. doi: 10.1128/jb.62.5.633-638.1951.
3
A simple and rapid assay of oxidative phosphorylation.一种简单快速的氧化磷酸化测定法。
Nature. 1955 Jun 25;175(4469):1120-1. doi: 10.1038/1751120a0.
4
THE ANTIBACTERIAL ACTIVITY OF SOME FLUORINE-CONTAINING BENZIMIDAZOLES.某些含氟苯并咪唑类化合物的抗菌活性
Biochem Pharmacol. 1964 May;13:751-4. doi: 10.1016/0006-2952(64)90011-5.
5
EFFECT OF IMIDAZOLE ON ADENOSINE TRIPHOSPHATASE, ADENOSINE TRIPHOSPHATE-INORGANIC PHOSPHATE EXCHANGE REACTION AND OXIDATIVE PHOSPHORYLATION.咪唑对三磷酸腺苷酶、三磷酸腺苷-无机磷酸交换反应及氧化磷酸化的影响
Biochim Biophys Acta. 1964 Mar 9;81:587-90. doi: 10.1016/0926-6569(64)90143-9.
6
IMIDAZOLE INHIBITION OF THE ENERGY-LINKED REDUCTION OF NAD+ BY SUCCINATE.咪唑对琥珀酸使NAD⁺进行能量偶联还原反应的抑制作用。
Biochim Biophys Acta. 1964 Mar 9;81:585-7. doi: 10.1016/0926-6569(64)90142-7.
7
PROTEIN AS THE MITOCHONDRIAL SITE FOR ACTION OF UNCOUPLING PHENOLS.蛋白质作为解偶联酚作用的线粒体位点。
Science. 1964 Aug 21;145(3634):824-6. doi: 10.1126/science.145.3634.824.
8
ON THE MECHANISM OF OXIDATIVE PHOSPHORYLATION. VII. THE ENERGY-REQUIRING REDUCTION OF PYRIDINE NUCLEOTIDE BY SUCCINATE AND THE ENERGY-YIELDING OXIDATION OF REDUCED PYRIDINE NUCLEOTIDE BY FUMARATE.论氧化磷酸化的机制。VII. 琥珀酸使吡啶核苷酸进行需能还原以及延胡索酸使还原型吡啶核苷酸进行产能氧化
Biochemistry. 1963 May-Jun;2:523-8. doi: 10.1021/bi00903a023.
9
Lipid solubility as a factor influencing the activity of uncoupling phenols.脂溶性作为影响解偶联酚活性的一个因素。
Biochim Biophys Acta. 1962 Sep 10;63:46-54. doi: 10.1016/0006-3002(62)90337-2.
10
New antimetabolites of vitamin B12.维生素B12的新型抗代谢物。
Nature. 1959 Oct 31;184:1383-4. doi: 10.1038/1841383a0.