Czeglédy J, Batár I, Evander M, Gergely L, Wadell G
Institute of Microbiology, University Medical School, Debrecen, Hungary.
Arch Gynecol Obstet. 1991;249(4):185-9. doi: 10.1007/BF02390386.
Exfoliated cells from the uterine cervix of 102 Hungarian women with no cytological abnormality were screened using the polymerase chain reaction (PCR) for human papillomavirus (HPV) type 16 infection. Twenty-nine patients with histologically confirmed cervical intraepithelial neoplasia (CIN) served as reference cases. PCR was performed with 2 different HPV 16 specific oligonucleotide primer pairs flanking a 300 and a 200 base-pair fragment from the early 6 (E6) and early 7 (E7) genes, position 215-514 and 605-805. The specimens exhibited the same proportions of type 16 sequences specific for the tested regions. 8.8% (9/102) of normal samples showed amplification for HPV type 16 E6 and E7 regions, while 48.3% (14/29) of CIN biopsies were positive for the same gene sequences.
利用聚合酶链反应（PCR）对102名细胞学检查无异常的匈牙利女性子宫颈脱落细胞进行了16型人乳头瘤病毒（HPV）感染筛查。29例经组织学确诊的宫颈上皮内瘤变（CIN）患者作为对照病例。使用2对不同的HPV 16特异性寡核苷酸引物对进行PCR，这两对引物分别位于早期6（E6）和早期7（E7）基因中300和200个碱基对片段两侧，位置分别为215 - 514和605 - 805。标本中所检测区域的16型序列比例相同。8.8%（9/102）的正常样本显示HPV 16型E6和E7区域有扩增，而48.3%（14/29）的CIN活检组织中相同基因序列呈阳性。
