Crops Research Division, Agricultural Research Service, United States Department of Agriculture, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1968 Sep;43(9):1339-46. doi: 10.1104/pp.43.9.1339.
A classification system is presented to distinguish 3 corn nucleases-Ribonuclease I, Ribonuclease II, and Nuclease I-which were described in the first paper of this series. The 2 ribonucleases are specific for RNA, are endonucleases, and liberate purine and pyrimidine cyclic nucleotides from dinucleotide monophosphates as well as from RNA. Ribonuclease I and II hydrolyze the purine cyclic nucleotides to 3'-nucleotides, while Ribonuclease II may also act on the pyrimidine cyclic nucleotides. Ribonuclease II is best characterized by its molecular weight of 17,000, by a higher pH optimum than Ribonuclease I, and by its absorption onto microsomes.Nuclease I is a partially purified endonuclease which produces 5'-nucleotides from RNA, DNA, and dinucleotide monophosphates. The same enzyme may also be a 3'-nucleotidase.The corn nucleases were compared with nucleases found in other plant species.
本文提出了一个分类系统，用于区分三种玉米核酸酶——核糖核酸酶 I、核糖核酸酶 II 和核酸酶 I——它们在本系列的第一篇论文中被描述。这两种核糖核酸酶对 RNA 具有特异性，是内切核酸酶，能够从二核苷酸单磷酸以及 RNA 中释放嘌呤和嘧啶环核苷酸。核糖核酸酶 I 和 II 将嘌呤环核苷酸水解为 3'-核苷酸，而核糖核酸酶 II 也可能作用于嘧啶环核苷酸。核糖核酸酶 II 的分子量为 17000，最适 pH 值高于核糖核酸酶 I，并且能被微粒体吸附，因此其特征性最强。核酸酶 I 是一种部分纯化的内切核酸酶，能够从 RNA、DNA 和二核苷酸单磷酸中产生 5'-核苷酸。同一种酶也可能是 3'-核苷酸酶。玉米核酸酶与其他植物物种中发现的核酸酶进行了比较。
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