Kole R, Sierakowska H, Szemplińska H, Shugar D
Nucleic Acids Res. 1974 May;1(5):699-706. doi: 10.1093/nar/1.5.699.
Mung bean nuclease hydrolyzes synthetic esters of 3'-nucleotides to nucleosides and phosphate esters; esters of 2'-nucleotides, and 2'--> 5' internucleotide linkages, are resistant. Esters of ribonucleotides are cleaved at 100-fold the rate for deoxyribonucleotides, the increased rate being due to presence of the 2'-hydroxyl and not to differences in conformation. Introduction of a 5'-substituent leads to a 3-fold increase in rate. The rates of hydrolysis vary up to 10-fold with the nature of the base, in the order adenine > hypoxanthine > uracil; and up to 6-fold with the nature of the ester radical. This form of cleavage of esters of 3'-nucleotides is also characteristic for nuclease-3'-nucleotidase activities from potato tubers and wheat, suggesting that one type of enzyme is responsible for all these activities.
绿豆核酸酶可将3'-核苷酸的合成酯水解为核苷和磷酸酯；2'-核苷酸的酯以及2'→5'核苷酸间连接键具有抗性。核糖核苷酸酯的切割速率是脱氧核糖核苷酸的100倍，速率增加是由于2'-羟基的存在，而非构象差异。引入5'-取代基会使速率提高3倍。水解速率随碱基性质变化可达10倍，顺序为腺嘌呤＞次黄嘌呤＞尿嘧啶；随酯基性质变化可达6倍。这种3'-核苷酸酯的切割形式也是马铃薯块茎和小麦中核酸酶-3'-核苷酸酶活性的特征，表明一种类型的酶负责所有这些活性。
