Beyer R E, Peters G A, Ikuma H
Laboratory of Chemical Biology, Department of Zoology, The University of Michigan, Ann Arbor, Michigan 48104.
Plant Physiol. 1968 Sep;43(9):1395-400. doi: 10.1104/pp.43.9.1395.
A procedure for the isolation of submitochondrial particles in quantity from etiolated Mung bean (Phaseolus aureus) seedlings is described. Using a combination of acetone extraction and 2 systems of thin layer chromatography ubiquinone has been isolated. The isolated ubiquinone migrates coincident with authentic ubiquinone-10 in reversed phase thin layer partition chromatography, gives a positive Craven's test, and has oxidized and reduced spectra characteristic of ubiquinone. The quinone is partially reduced under steady-state electron transfer conditions with both succinate and NADH as substrates and is almost completely reduced under anaerobic conditions with either substrate. The concentration of ubiquinone in the particle is of the order of 4.4 mmumoles per mg particle protein, approximately equal to that found in similar submitochondrial particles from beef heart. It is tentatively concluded that ubiquinone-10 is a functional member of the mitochondrial electron transfer chain of Phaseolus aureus.
本文描述了一种从黄化绿豆（Phaseolus aureus）幼苗中大量分离亚线粒体颗粒的方法。通过丙酮萃取和两种薄层层析系统相结合，已分离出泛醌。在反相薄层层析中，分离出的泛醌与标准泛醌-10迁移一致，对克雷文氏试验呈阳性反应，并且具有泛醌的氧化和还原光谱特征。在以琥珀酸和NADH作为底物的稳态电子转移条件下，该醌会部分还原，而在以任一底物进行厌氧条件下时，它几乎会完全还原。颗粒中泛醌的浓度约为每毫克颗粒蛋白4.4微摩尔，这与从牛心中分离得到的类似亚线粒体颗粒中的浓度大致相当。初步得出结论，泛醌-10是绿豆线粒体电子传递链的一个功能成员。
