Department of Biochemistry and Biophysics, University of California, San Francisco, California 94122.
Plant Physiol. 1973 Dec;52(6):622-6. doi: 10.1104/pp.52.6.622.
The effect of various agents on the activation of succinate dehydrogenase in cauliflower (Brassica oleracea) and mung bean (Phaseolus aureus) mitochondria and in sonicated particles has been investigated. Reduced coenzyme Q(10), inosine diphosphate, inosine triphosphate, acid pH, and anions activate the enzyme in mitochondria from higher plants in the same manner as in mammalian preparations. Significant differences have been detected in the behavior of plant and animal preparations in the effects of ATP, ADP, NADH, NAD-linked substrates, and of 2, 4-dinitrophenol on the state of activation of the dehydrogenase. In mammalian mitochondria ATP activates, whereas ADP does not, and the ATP effect is shown only in intact mitochondria. In mung bean and cauliflower mitochondria, both ATP and ADP activate and the effect is also shown in sonicated and frozen-thawed preparations. In sonicated mung bean mitochondria NADH causes complete activation, as in mammalian submitochondrial particles, but in sonicated cauliflower mitochondria activation by NADH is incomplete, as is also true of intact, anaerobic cauliflower mitochondria. Moreover, neither NAD-linked substrates nor a combination of these with NADH can fully activate the enzyme in cauliflower mitochondria. In contrast to mammalian mitochondria, succinate dehydrogenase is not deactivated in cauliflower or mung beam mitochondria under the oxidized conditions brought about by uncoupling of oxidative phosphorylation by 2,4-dinitrophenol.
已研究了各种试剂对花椰菜（ Brassica oleracea ）和绿豆（ Phaseolus aureus ）线粒体中琥珀酸脱氢酶以及超声处理颗粒中琥珀酸脱氢酶的激活作用。还原辅酶 Q(10)、肌苷二磷酸、肌苷三磷酸、酸性 pH 值和阴离子以与哺乳动物制剂相同的方式激活高等植物线粒体中的酶。在植物和动物制剂对 ATP、ADP、NADH、NAD 连接底物以及 2,4-二硝基苯酚对脱氢酶激活状态的影响方面，已检测到明显的差异。在哺乳动物线粒体中，ATP 激活，但 ADP 不激活，并且仅在完整线粒体中显示 ATP 效应。在绿豆和花椰菜线粒体中，ATP 和 ADP 均能激活，并且该效应也在超声处理和冷冻-解冻制剂中显示。在超声处理的绿豆线粒体中，NADH 引起完全激活，就像在哺乳动物亚线粒体颗粒中一样，但在超声处理的花椰菜线粒体中，NADH 的激活不完全，与无氧花椰菜线粒体的情况相同。此外，NAD 连接的底物或这些底物与 NADH 的组合均不能使花椰菜线粒体中的酶完全激活。与哺乳动物线粒体不同，在由 2,4-二硝基苯酚解偶联氧化磷酸化引起的氧化条件下，花椰菜或绿豆线粒体中的琥珀酸脱氢酶不会失活。
