关于小麦幼苗活性、紧密偶联线粒体分离方法的研究。
On methods of isolation of active, tightly coupled mitochondria of wheat seedlings.
机构信息
Institute of Life Science, Texas A & M University, College Station, Texas 77843.
出版信息
Plant Physiol. 1968 Sep;43(9):1406-10. doi: 10.1104/pp.43.9.1406.
Abstract
Tightly coupled mitochondria can be isolated from wheat seedlings 1) if young seedlings are used, 2) if the grinding medium is buffered and contains bovine serum albumin (BSA), 3) if the isolation procedure, including centrifugation is 10 to 11 minutes long. Mitochondria isolated in this manner oxidize alpha-ketoglutarate, malate and NADH. Respiratory control (R.C.) values are around 6, 3, and 10, respectively. ADP:O values with those substrates are 4.0, 2.9, and 1.7, respectively. Mitochondria are stable for 3 hours. It is suggested that the technique may be used for preparation of mitochondria for comparative studies since activities of these mitochondria are highly reproducible.
摘要
如果使用幼嫩的麦苗、在研磨介质中缓冲并添加牛血清白蛋白（BSA）、且整个分离过程（包括离心步骤）持续 10-11 分钟，那么可以从小麦幼苗中分离出紧密结合的线粒体。以这种方式分离出的线粒体可以氧化α-酮戊二酸、苹果酸和 NADH。呼吸控制（R.C.）值分别约为 6、3 和 10。这些底物的 ADP:O 值分别为 4.0、2.9 和 1.7。线粒体在 3 小时内保持稳定。建议该技术可用于为比较研究制备线粒体，因为这些线粒体的活性具有高度可重复性。
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