Department of Botany, University of California, Davis, California 95616.
Plant Physiol. 1968 Oct;43(10):1597-604. doi: 10.1104/pp.43.10.1597.
A rapid oxygraph method of studying the permeability of the envelope of isolated chloroplasts was used. The outer envelope of aqueously isolated whole spinach (Spinacia oleracea L.) chloroplasts in buffer is readily permeable to 3-phosphoglyceric acid, which induces an immediate light dependent oxygen evolution. This light dependent oxygen evolution was completely eliminated by swelling these plastids in an osmotically dilute solution. Exogenous adenosine diphosphate, but not inorganic phosphate, strongly stimulated this oxygen evolution. This indicated that the chloroplast envelope is relatively permeable to adenosine diphosphate.Oxygen evolution and swelling studies indicated that the chloroplast envelope is relatively impermeable to NADP and to ferredoxin.A method is described whereby the percent of whole chloroplasts present in a chloroplast preparation may be rapidly estimated.
采用一种快速氧测定法来研究分离叶绿体的被膜通透性。在缓冲液中用水分离的完整菠菜（Spinacia oleracea L.）叶绿体的外被膜对 3-磷酸甘油酸具有很高的通透性，后者诱导立即发生依赖于光的氧释放。用渗透压稀溶液使这些叶绿体膨胀，可完全消除这种依赖于光的氧释放。外加的二磷酸腺苷，但不是无机磷，强烈地刺激这种氧释放。这表明叶绿体被膜对二磷酸腺苷具有相对高的通透性。氧释放和膨胀研究表明，叶绿体被膜对 NADP 和铁氧还蛋白的通透性较低。本文描述了一种快速估计叶绿体制剂中完整叶绿体百分数的方法。
