Department of Biology, State University of New York, Buffalo, New York 14214.
Plant Physiol. 1968 Oct;43(10):1710-6. doi: 10.1104/pp.43.10.1710.
When Wolffiella floridana, an aquatic angiosperm in the family, Lemnaceae, was grown in axenic culture under continuous light in E medium containing 1.0% sucrose and a micromolar amount of (14)C-labeled myo-inositol (MI), MI was taken up by the growing plants and converted to phytic acid. After 13 weeks in labeled medium during which time there was a 1000-fold increase in fresh weight, 30% of the (14)C was recovered in ethanol insoluble residue. Extraction of this residue with EDTA released 70% of the label into solution. Phytic acid, identified by paper electrophoresis, ion exchange chromatography, and hydrolysis with phytase, accounted for most of this radioactivity although some label was also found in pentaphosphate and lower phosphate esters of MI. Very little MI was converted to cell wall polysaccharides under the conditions used. Results of this study indicate that Wolffiella floridana is a convenient tissue for the study of phytic acid biosynthesis under laboratory conditions.Lemna gibba G3, grown under short day conditions in medium of the same composition as that used for W. floridana, also formed labeled phytic acid as well as other labeled lower phosphate esters of MI.
当水生被子植物浮萍科浮萍属的佛罗里达浮萍在含有 1.0%蔗糖和微量 (14)C 标记肌醇 (MI) 的 E 培养基中在持续光照下进行无菌培养时，MI 被生长中的植物吸收并转化为植酸。在标记培养基中培养 13 周后，植物鲜重增加了 1000 倍，其中 30%的 (14)C 回收于乙醇不溶残渣中。用 EDTA 提取该残渣可将 70%的标记物释放到溶液中。通过纸电泳、离子交换色谱和植酸酶水解鉴定出植酸，它占了大部分放射性物质，尽管也在五磷酸盐和 MI 的低磷酸盐酯中发现了一些标记物。在使用的条件下，MI 很少转化为细胞壁多糖。本研究结果表明，佛罗里达浮萍是在实验室条件下研究植酸生物合成的一种方便组织。在与用于 W. floridana 的相同组成的培养基中，短日条件下生长的浮萍 G3 也形成了标记植酸以及 MI 的其他标记低磷酸盐酯。
