Brearley C A, Hanke D E
Department of Plant Sciences, University of Cambridge, U.K.
Biochem J. 1996 Feb 15;314 ( Pt 1)(Pt 1):215-25. doi: 10.1042/bj3140215.
We have undertaken an analysis of the inositol phosphates of Spirodela polyrhiza at a developmental stage when massive accumulation of InsP6 indicates that a large net synthesis is occurring. We have identified Ins3P, Ins(1,4)P2, Ins(3,4)P2 and possibly Ins(4,6)P2, Ins(3,4,6)P3, Ins(3,4,5,6)P4, Ins (1,3,4,5,6)P5, D- and/or L-Ins(1,2,4,5,6)P5 and InsP6 and revealed the likely presence of a second InsP3 with chromatographic properties similar to Ins(1,4,5)P3. The higher inositol phosphates identified show no obvious direct link to pathways of metabolism of second messengers purported to operate in higher plants, nor do they resemble the immediate products of plant phytase action on InsP6.
我们对多根紫萍发育阶段的肌醇磷酸进行了分析,在该阶段,InsP6的大量积累表明正在发生大量的净合成。我们已鉴定出Ins3P、Ins(1,4)P2、Ins(3,4)P2,可能还有Ins(4,6)P2、Ins(3,4,6)P3、Ins(3,4,5,6)P4、Ins(1,3,4,5,6)P5、D-和/或L-Ins(1,2,4,5,6)P5以及InsP6,并揭示可能存在第二种具有与Ins(1,4,5)P3相似色谱特性的InsP3。所鉴定出的较高肌醇磷酸与据称在高等植物中起作用的第二信使代谢途径没有明显的直接联系,它们也不像植物植酸酶作用于InsP6的直接产物。