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大豆[大豆（Glycine max (L.) Merr.）]中的ATP硫酸化酶活性。

ATP Sulfurylase Activity in the Soybean [Glycine max (L.) Merr.].

作者信息

Adams C A, Johnson R E

机构信息

United States Regional Soybean Laboratory, University of Illinois, Urbana, Illinois 61801.

出版信息

Plant Physiol. 1968 Dec;43(12):2041-4. doi: 10.1104/pp.43.12.2041.

DOI:10.1104/pp.43.12.2041
PMID:16657005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1087122/
Abstract

ATP sulfurylase activity was assayed in soybean leaf extracts. A simple, rapid assay system using molybdate as an analogue of sulfate was developed. The assay was coupled to inorganic pyrophosphatase. The high pyrophosphatase level in soybean leaf extracts obviated the necessity of adding this enzyme to the assay system. ATP sulfurylase has a pH maximum above 7.5, uses molybdate and ATP as substrates, and requires magnesium ions for activity.

摘要

在大豆叶片提取物中测定了ATP硫酸化酶活性。开发了一种使用钼酸盐作为硫酸盐类似物的简单、快速的测定系统。该测定与无机焦磷酸酶偶联。大豆叶片提取物中高焦磷酸酶水平消除了在测定系统中添加该酶的必要性。ATP硫酸化酶在pH高于7.5时活性最高，以钼酸盐和ATP为底物，并且需要镁离子来发挥活性。

相似文献

1
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2
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引用本文的文献

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Plant Physiol. 1979 Oct;64(4):590-3. doi: 10.1104/pp.64.4.590.
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本文引用的文献

1
The role of adenosine-5'-phosphosulfate in the reduction of sulfate to sulfite by Desulfovibrio desulfuricans.腺苷-5'-磷酸硫酸在脱硫脱硫弧菌将硫酸盐还原为亚硫酸盐过程中的作用。
J Biol Chem. 1962 Jan;237:198-203.
2
THE CONTROL OF SULPHATE ACTIVATION IN BACTERIA.细菌中硫酸盐激活的调控
Biochem J. 1965 Jul;96(1):276-80. doi: 10.1042/bj0960276.
3
Metabolism of sulfur compounds (sulfate metabolism).硫化合物的代谢（硫酸盐代谢）。
Annu Rev Biochem. 1960;29:347-64. doi: 10.1146/annurev.bi.29.070160.002023.
4
Purification and properties of ATP-sulphate adenylyltransferase from liver.
Biochim Biophys Acta. 1968 Mar 25;151(3):725-7. doi: 10.1016/0005-2744(68)90032-6.