Botany Department, La Trobe University, Bundoora, Vic. 3083, Australia.
Plant Physiol. 1971 Jan;47(1):114-8. doi: 10.1104/pp.47.1.114.
The sulfate-dependent pyrophosphate exchange reaction has been re-examined and confirmed. Standard assay conditions for measuring ATP sulfurylase by sulfate-dependent pyrophosphate exchange are described and some properties of the enzyme (measured in crude dialyzed extracts) are reported. This method has many advantages over the well established molybdate method.
硫酸盐依赖的焦磷酸交换反应已经被重新检验和确认。通过硫酸盐依赖的焦磷酸交换来测量 ATP 硫酸化酶的标准测定条件被描述,并且该酶的一些性质(在粗透析提取物中测量)被报道。该方法比成熟的钼酸盐法具有许多优势。