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用焦磷酸交换法测定腺苷 5′-三磷酸硫酸化酶。

Assay of adenosine 5-triphosphate sulfurylase by pyrophosphate exchange.

机构信息

Botany Department, La Trobe University, Bundoora, Vic. 3083, Australia.

出版信息

Plant Physiol. 1971 Jan;47(1):114-8. doi: 10.1104/pp.47.1.114.

DOI:10.1104/pp.47.1.114
PMID:16657565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365822/
Abstract

The sulfate-dependent pyrophosphate exchange reaction has been re-examined and confirmed. Standard assay conditions for measuring ATP sulfurylase by sulfate-dependent pyrophosphate exchange are described and some properties of the enzyme (measured in crude dialyzed extracts) are reported. This method has many advantages over the well established molybdate method.

摘要

硫酸盐依赖的焦磷酸交换反应已经被重新检验和确认。通过硫酸盐依赖的焦磷酸交换来测量 ATP 硫酸化酶的标准测定条件被描述，并且该酶的一些性质（在粗透析提取物中测量）被报道。该方法比成熟的钼酸盐法具有许多优势。

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引用本文的文献

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本文引用的文献

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The estimation of phosphorus.磷的测定
Biochem J. 1940 Jun;34(6):858-65. doi: 10.1042/bj0340858.
2
Influence of age and sulfur metabolism on ATP sulfurylase activity in the soybean and a survey of selected species.年龄和硫代谢对大豆中ATP硫酸化酶活性的影响以及部分选定物种的调查
Plant Physiol. 1969 Sep;44(9):1241-6. doi: 10.1104/pp.44.9.1241.
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ATP Sulfurylase Activity in the Soybean [Glycine max (L.) Merr.].大豆[大豆（Glycine max (L.) Merr.）]中的ATP硫酸化酶活性。
Plant Physiol. 1968 Dec;43(12):2041-4. doi: 10.1104/pp.43.12.2041.
4
Amino acid dependent exchange between pyrophosphate and adenosine triphosphate in spinach preparations.菠菜制品中焦磷酸与三磷酸腺苷之间的氨基酸依赖性交换
J Biol Chem. 1959 May;234(5):1238-40.
5
Enzymatic reactions involving sulfate, sulfite, selenate, and molybdate.涉及硫酸盐、亚硫酸盐、硒酸盐和钼酸盐的酶促反应。
J Biol Chem. 1958 Oct;233(4):975-81.
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The isolation of a tryptophan-activating enzyme from pancreas.从胰腺中分离出一种色氨酸激活酶。
Arch Biochem Biophys. 1956 Nov;65(1):21-38. doi: 10.1016/0003-9861(56)90173-4.