3,5-二卤代-4-羟基苯甲腈对马铃薯块茎线粒体活性的影响。
Effects of 3,5-Dihalogenated-4-hydroxybenzonitriles on the Activity of Mitochondria From White Potato Tubers.
机构信息
Departments of Crop Science and Botany, North Carolina State University, Raleigh, North Carolina 27607.
出版信息
Plant Physiol. 1969 Mar;44(3):429-34. doi: 10.1104/pp.44.3.429.
Effects of the herbicide 3,5-diiodo-4-hydroxybenzonitrile (ioxynil), and its dibromo and dichloro analogs, upon the respiration of mitochondria isolated from white potato tubers (Solanum tuberosum L.) were investigated. Mitochondrial-mediated oxygen uptake was monitored polarographically with a platinum oxygen electrode.With succinate as substrate for mitochondrial oxidation, ioxynil stimulated both ADP-limited and ADP + phosphate-deficient oxygen utilization. Ioxynil also circumvented oligomycin-inhibited respiration and slightly stimulated mitochondrial ATPase activity. Responses produced by ioxynil on respiration were similar to, but occurred at lower concentrations than, those of 2,4-dinitrophenol.The dibromo- and dichloro-substituted analogs of ioxynil also stimulated ADP-limited respiration and released oligomycin-inhibited oxygen uptake. Based on concentration, the decreasing order of uncoupling activity was I > Br > Cl with respect to the halogen substituents.
研究了除草剂 3,5-二碘-4-羟基苯甲腈（碘苯腈）及其二溴和二氯类似物对分离自白土豆（Solanum tuberosum L.）的线粒体呼吸的影响。使用白金氧电极通过极谱法监测线粒体介导的耗氧量。
以琥珀酸盐作为线粒体氧化的底物，碘苯腈刺激 ADP 限制和 ADP + 磷酸盐缺乏的氧气利用。碘苯腈还绕过寡霉素抑制的呼吸并轻微刺激线粒体 ATP 酶活性。碘苯腈对呼吸产生的反应与 2,4-二硝基苯酚的反应相似，但在较低浓度下发生。碘苯腈的二溴和二氯取代类似物也刺激 ADP 限制呼吸并释放寡霉素抑制的耗氧量。基于浓度，对于卤素取代基，解偶联活性的降序为 I > Br > Cl。
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