短期流入作为跨质膜流入的一种衡量指标。
Short term influx as a measure of influx across the plasmalemma.
作者信息
Cram W J
机构信息
Department of Botanical Sciences, University of California, Los Angeles, California 90024.
出版信息
Plant Physiol. 1969 Jul;44(7):1013-5. doi: 10.1104/pp.44.7.1013.
Abstract
It is shown that the influx of tracer to a plant cell must be measured for less than one third of the half-time for exchange of cytoplasmic tracer, and any subsequent wash must be negligibly brief, if the initial influx measured over a finite period is to be a good estimate of the plasmalemma influx. Complications due to lack of knowledge of the cytoplasmic exchange rate constant and to extracellular contents make it difficult to make such an estimate from influx measurements alone. The use of influx measurements is further discussed.
摘要
结果表明，如果要使在有限时间段内测得的初始流入量能很好地估计质膜流入量，那么示踪剂流入植物细胞的测量时间必须少于细胞质示踪剂交换半衰期的三分之一，并且任何后续冲洗时间都必须短到可以忽略不计。由于对细胞质交换速率常数缺乏了解以及细胞外成分的影响，仅通过流入量测量很难做出这样的估计。文中进一步讨论了流入量测量的应用。
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